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Protein Hydrolysates from Citrullus lanatus Seed: Antiradical and Hydrogen Peroxide-scavenging properties and kinetics of Angiotensin-I converting enzyme inhibition

Authors:

R. O. Arise ,

University of Ilorin, P.M.B. 1515, Ilorin, NG
About R. O.
Department of Biochemistry, Faculty of Life Sciences
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A. A. Yekeen,

University of Ilorin, P.M.B. 1515, Ilorin, NG
About A. A.
Department of Biochemistry, Faculty of Life Sciences
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O. E. Ekun,

Adekunle Ajasin University, Akungba-Akoko, NG
About O. E.
Department of Biochemistry, Faculty of Science
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O. J. Olatomiwa

University of Ilorin, P.M.B. 1515, Ilorin, NG
About O. J.
Department of Biochemistry, Faculty of Life Sciences
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Abstract

This study investigated the in vitro antihypertensive, antiradical and hydrogen peroxide-scavenging properties of protein hydrolysates from Citrullus lanatus (watermelon) seed (CSPHs) obtained through enzymatic digestion. Proteins from watermelon seeds were isolated and enzymatically hydrolyzed with non-specific (alcalase), moderately specific (pepsin) and highly specific (trypsin) proteases, mimicking human gastrointestinal digestion. The hydrolysates were investigated for inhibitory property against angiotensin-I-converting enzyme (ACE) activity. Using N-[3-(2-furyl)acryloyl]-L-phenylalanyl-glycyl-glycine as the substrate, CSPHs showed concentration-dependent ACE inhibition (IC50 1.377 - 1.757 mg/mL) with peptic CSPH having the strongest ACE-inhibition followed by tryptic CSPH. Kinetic analysis revealed that peptic CSPH inhibited ACE activity in a mixed-type inhibition pattern while alcalase and tryptic CSPHs exhibited non-competitive inhibition mode. Peptic CSPH demonstrated the strongest DPPH radical-scavenging activity while tryptic CSPH showed the highest H2O2-scavenging property. These results show that protein hydrolysates from watermelon seed possess bioactivities that could be exploited in the management of hypertension.
How to Cite: Arise, R. O., Yekeen, A. A., Ekun, O. E., & Olatomiwa, O. J. (2016). Protein Hydrolysates from Citrullus lanatus Seed: Antiradical and Hydrogen Peroxide-scavenging properties and kinetics of Angiotensin-I converting enzyme inhibition. Ceylon Journal of Science, 45(2), 39–52. DOI: http://doi.org/10.4038/cjs.v45i2.7387
Published on 13 Sep 2016.
Peer Reviewed

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