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The hexane-ethyl acetate-methanol-water system for the separation of theaflavins from black tea (Camellia sinensis) using high-speed counter-current chromatography

Authors:

N. Savitri Kumar ,

Institute of Fundamental Studies (IFS), Hantana Road, Kandy, LK
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P. A. Nimal Punyasiri,

Tea Research Institute, Talawakelle, LK
About P. A. Nimal
Biochemistry Division
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W. A. M. B. Wijekoon

University of Peradeniya, LK
About W. A. M. B.
Department of Chemistry
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Abstract

Theaflavins (TFs) from black tea leaves were separated using high-speed countercurrent chromatography (HSCCC). Isobutyl methyl ketone (4-methyl-2-pentanone) was used to separate the TFs from a hot water extract of black tea leaves. Three different compositions of the biphasic solvent system hexane–ethyl acetate-methanol-water (2:5:2:5 solvent system A) 1:4:1:4 (solvent system B) and 1:5:1:5 (solvent system C) were studied. The aqueous phase was used as the mobile phase in the head to tail elution mode. Flow rates of 2.0 – 2.8 mL min-1, revolution speeds of 800-1000 rpm and a 200 mg sample of TF in 5 mL of the aqueous phase with settling times 17-18 secs (solvent systems B and C) gave the best separations. Three peaks were separated in the HSCCC traces obtained using solvent systems B and C, and each peak was monitored by high performance liquid chromatography (HPLC).Separation of theaflavin monogallates was not achieved, but solvent system Cresulted in a complete separation of theaflavin and theaflavin digallate.
How to Cite: Kumar, N. S., Punyasiri, P. A. N., & Wijekoon, W. A. M. B. (2016). The hexane-ethyl acetate-methanol-water system for the separation of theaflavins from black tea (Camellia sinensis) using high-speed counter-current chromatography. Ceylon Journal of Science, 45(2), 79–86. DOI: http://doi.org/10.4038/cjs.v45i2.7391
Published on 13 Sep 2016.
Peer Reviewed

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