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Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in Bacopa monnieri(L.)

Authors:

Poornananda Madhava Naik,

King Faisal University, P.O. Box 420, Al-Hassa 31982, SA
About Poornananda Madhava
Department of Agricultural Biotechnology, College of Agricultural and Food Sciences
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Manoj Godbole ,

Sri Dharmasthala Manjunatheshwara College (Autonomous), Ujire -574240, IN
About Manoj
PG Department of Biotechnology
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Praveen Nagella,

Christ University, Hosur Road, Bengaluru – 560029, IN
About Praveen
Department of Botany
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Hosakatte Niranjana Murthy

Karnatak University, Dharwad – 560003, IN
About Hosakatte Niranjana
PG Department of Botany
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Abstract

Bacopa monnieri (L.) Wettst. (Plantaginaceae), commonly known as Brahmi, is used in Ayurveda as a medicinal herb, found throughout the Indian subcontinent in wet, damp and marshy areas. The herb yields pharmaceutically active compounds called bacosides. The current investigation was carried out to assess the potential for increasing biomass and the concentration of bacoside A from the in vitro regenerated shoots in different media (MS, B5, NN and N6), the strength of the MS medium (0.25, 0.50, 0.75, 1.0, 1.50 and 2.0X) and carbon source [sucrose, glucose, fructose, maltose, sucrose + glucose (1:1), sucrose + maltose (1:1), glucose + fructose (1:1), glucose + maltose (1:1), fructose + sucrose (1:1) and fructose + maltose (1:1)]. All the culture media were supplemented with 2 mg/L Kinetin (Kin) at pH 5.8. High performance liquid chromatography (HPLC) was used to determine the bacoside A content from the regenerated shoots. Optimum number of adventitious shoots (70.75 shoots per explant), fresh weight (2.344 g), dry weight (0.166 g) and bacoside A content (13.052 mg/g DW) were obtained from the cultures grown in the MS medium. Bacoside A production was highest in the shoots grown in the glucose + fructose combination (15.588 mg/g DW) among the different carbon sources tested. These findings suggest that B. monnieri culture requires full strength MS medium and a moderate level of sucrose. The present study is useful to obtain optimum production of biomass and bacoside A from the in vitro regenerated shoots of B. monnieri.
How to Cite: Naik, P. M., Godbole, M., Nagella, P., & Murthy, H. N. (2017). Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in Bacopa monnieri(L.). Ceylon Journal of Science, 46(4), 97–104. DOI: http://doi.org/10.4038/cjs.v46i4.7472
Published on 12 Dec 2017.
Peer Reviewed

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