Start Submission Become a Reviewer

Reading: Analyses of phylogenetics, starch granule morphology and consumer preference of Canna indica...

Download

A- A+
Alt. Display

Research Articles

Analyses of phylogenetics, starch granule morphology and consumer preference of Canna indica L. grown in Sri Lanka

Authors:

R. W. K. M. Senevirathna,

University of Peradeniya, Peradeniya, LK
About R. W. K. M.
Department of Molecular Biology and Biotechnology, Faculty of Science
X close

L. T. Ranaweera,

University of Peradeniya, Peradeniya, LK
About L. T.
Department of Molecular Biology and Biotechnology, Faculty of Science
X close

N. D. U. S. Nakandala,

University of Peradeniya, Peradeniya, LK
About N. D. U. S.
Department of Molecular Biology and Biotechnology, Faculty of Science
X close

H. M. T. N. Senavirathna,

University of Peradeniya, Peradeniya, LK
About H. M. T. N.
Department of Molecular Biology and Biotechnology, Faculty of Science
X close

W. M. D. A. Wijesundara,

University of Peradeniya, Peradeniya, LK
About W. M. D. A.
Department of Molecular Biology and Biotechnology, Faculty of Science
X close

H. S. M. Jayarathne,

University of Peradeniya, Peradeniya, LK
About H. S. M.
Department of Molecular Biology and Biotechnology, Faculty of Science
X close

C. K. Weebadde,

Michigan State University, East Lansing, 48824, US
About C. K.
Department of Plant, Soil and Microbial Sciences, College of Agriculture and Natural Resources
X close

S. D. S. S. Sooriyapathirana

University of Peradeniya, Peradeniya, LK
About S. D. S. S.

Department of Molecular Biology and Biotechnology, Faculty of Science

 

Postgraduate Institute of Science, University of Peradeniya, Peradeniya

X close

Abstract

Canna indica is a tuber crop which has many medicinal values. In Sri Lanka, C. indica tubers are consumed in rural areas and mainly available in street-markets of Nuwara-Eliya and Kandy Districts. In the present study, we assessed the phylogenetics of C. indica, starch granule morphology and consumer preference of C. indica tubers in comparison to the popular tuber crops. The phylogenetic analysis was conducted based on the sequence polymorphism at rbcL, atpB gene, trnL-trnF and trnH-psbA marker-loci with respect to the ornamental Canna spp. in Sri Lanka and the previously published sequences of Canna spp. The starch granules were isolated and observed under optical and scanning electron microscopes. The diameter and the surface area of the starch granules were measured under the optical microscope and subjected to analysis of variance. As C. indica tubers are consumed as boiled tuber pieces in Sri Lanka, the consumer preference analysis was conducted using the boiled tuber pieces C. indica, Xanthosoma sagittifolium, Manihot esculenta, Solanum tuberosum, and Ipomoea batatas. The phylogenetic tree based on rbcL marker revealed that C. indica in Sri Lanka is slightly divergent from the other Canna spp. Only the polymorphism of the atpB gene can be used to differentiate C. indica from the ornamental Canna sp. in Sri Lanka. The morphological analysis of starch granules revealed that C. indica has the biggest scallop-seashell shaped starch granules compared to other tuber species. The boiled C. indica tubers were accepted better than that of X. sagittifolium, rated equally to the tubers of S. tuberosum and M. esculenta, and rated less than I. batatas. The hardy and fibrous nature of C. indica tubers must be the major limiting factors for achieving the highest consumer preference highlighting the need of breeding for better texture in tubers
How to Cite: Senevirathna, R. W. K. M., Ranaweera, L. T., Nakandala, N. D. U. S., Senavirathna, H. M. T. N., Wijesundara, W. M. D. A., Jayarathne, H. S. M., … Sooriyapathirana, S. D. S. S. (2020). Analyses of phylogenetics, starch granule morphology and consumer preference of Canna indica L. grown in Sri Lanka. Ceylon Journal of Science, 49(3), 261–273. DOI: http://doi.org/10.4038/cjs.v49i3.7777
Published on 25 Sep 2020.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus