Start Submission Become a Reviewer

Reading: Molecular phylogeny-based identification of Colletotrichum endophytica and C. siamense as c...

Download

A- A+
Alt. Display

Research Articles

Molecular phylogeny-based identification of Colletotrichum endophytica and C. siamense as causal agents of avocado anthracnose in Sri Lanka

Authors:

D. M. S. Dissanayake,

University of Peradeniya, Peradeniya, LK
About D. M. S.
Department of Botany
X close

N. K. B. Adikaram ,

University of Peradeniya, Peradeniya, LK
About N. K. B.

Department of Botany

 

National Institute of Fundamental Studies, Hantana Road, Kandy

X close

D. M. D. Yakandawala,

University of Peradeniya, Peradeniya, LK
About D. M. D.
Department of Botany
X close

L. Jayasinghe

National Institute of Fundamental Studies, Hantana Road, Kandy, LK
X close

Abstract

Avocado (Persea americana) is a sub-tropical fruit with high nutritional value and numerous health benefits. Among the postharvest fungal diseases that affect ripe avocados, anthracnose is one of the most destructive disease worldwide, causing significant postharvest fruit losses and limiting shelf life. Over 15 Colletotrichum species have been reported as causing avocado anthracnose from avocado growing countries in the world. In the present study, 35 Colletotrichum isolates were obtained from ripe avocados showing anthracnose symptoms, collected from the Central and North Western Province of Sri Lanka. Fifteen randomly selected isolates were subjected to DNA sequence analysis using ITS, TUB2, and GAPDH regions. Species affiliations and identities of the resulting sequences were determined through similarity-based searches of the NCBI GenBank Database. Based on the combined phylogentic analysis of three gene regions, nine and six isolates were identified as C. endophytica and C. siamense respectively, both belonging to the C. gloeosporioides species complex. Of the two species, C. endophytica is reported as a causal agent of avocado anthracnose for the first time.
How to Cite: Dissanayake, D. M. S., Adikaram, N. K. B., Yakandawala, D. M. D., & Jayasinghe, L. (2021). Molecular phylogeny-based identification of Colletotrichum endophytica and C. siamense as causal agents of avocado anthracnose in Sri Lanka. Ceylon Journal of Science, 50(4), 449–458. DOI: http://doi.org/10.4038/cjs.v50i4.7943
Published on 15 Dec 2021.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus