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Research Articles

Modification of nutrient agar medium to culture yet-unculturable bacteria living in unsanitary landfills

Authors:

C. Devika,

Uva Wellassa University of Sri Lanka, Badulla, LK
About C.
Department of Science and Technology, Faculty of Applied Sciences
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I. D. Singhalage ,

Uva Wellassa University of Sri Lanka, Badulla, LK
About I. D.
Department of Science and Technology, Faculty of Applied Sciences
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G. Seneviratne

National Institute of Fundamental Studies, Kandy, LK
About G.
Microbial Biotechnology Unit
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Abstract

The microbial diversity in waste dumps is considered to be high. Generally, ca. 99% of bacterial species remain unculturable in standard media. This research was focused to modify Nutrient Agar (NA) mimicking the natural substrate where bacteria actually live. NA medium was modified by adding 40% (M1), 32% (M2), 24% (M3), 16% (M4), and 8% (M5) (v/v) soil extracts obtained from the dump site to isolate its bacteria. Conventional NA was the control. The isolates were characterized by FTIR spectroscopy. Seventy-eight and 18 bacterial strains were isolated from the modified media (M1-M5) and the control, respectively. Absorbance of the FTIR spectra constituted six clusters. According to the cluster analysis, the 100% similarity was observed in three bacterial couples, which were considered as three strains. Thus, the modified media of the present study facilitated the culturing of 75 unculturable bacterial strains over the conventional NA and percent improvement of isolation was 417% over NA. Results revealed the potential of extracted soil biochemicals and nutrients to modify the NA for culturing yet-unculturable bacteria living in unsanitary landfills.
How to Cite: Devika, C., Singhalage, I. D., & Seneviratne, G. (2021). Modification of nutrient agar medium to culture yet-unculturable bacteria living in unsanitary landfills. Ceylon Journal of Science, 50(4), 505–512. DOI: http://doi.org/10.4038/cjs.v50i4.7949
Published on 15 Dec 2021.
Peer Reviewed

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