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In vitro α-amylase inhibitory and antioxidant properties of pancreatin-derived Polyalthia longifolia seed protein hydrolysate

Authors:

O. T. Babatunde ,

Crown-Hill University, P.M.B 1605, Eyenkorin, Ilorin, Kwara State, NG
About O. T.

Biochemistry Unit, Department of Chemical Sciences, Faculty of Science

 

Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, P.M.B 1515, Ilorin, Kwara State, Nigeria

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A. Igunnu,

University of Ilorin, P.M.B 1515, Ilorin, Kwara State, NG
About A.
Department of Biochemistry, Faculty of Life Sciences
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C. O. Bewaji

University of Ilorin, P.M.B 1515, Ilorin, Kwara State, NG
About C. O.

Department of Biochemistry, Faculty of Life Sciences

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Abstract

Inhibition of α-amylase and free radical scavenging activities are two basic approaches utilized in the management of postprandial hyperglycemia. In recent times, researchers have explored the α-amylase inhibitory and free radical scavenging potentials of plant-derived peptides to circumvent the side effects of synthetic drugs. In this study, proteins from Polyalthia longifolia (P. longifoliawere isolated using alkaline solubilisation-acid precipitation method and the proteins were enzymatically hydrolyzed with pancreatin. The hydrolysate derived was investigated for α-amylase inhibitory property, using starch as the substrate; and antioxidant property, using 2, 20-azinobis-3-ethylbenzothiazoline-6-sulphonate cation radical (ABTS+) scavenging and ferric ion reducing antioxidant power (FRAP) assays. The hydrolysate demonstrated a concentration- dependentα-amylaseinhibitorypotential(IC506.49±0.08mg/ml) exhibiting 64.40 ± 5.58% α-amylase inhibition at the highest concentration considered (9 mg/ml). Kinetics analyses revealed that the hydrolysate exhibited uncompetitive inhibition pattern on α-amylase at 9 mg/ml. The hydrolysate exhibited considerable radical scavenging activity (85.11 ± 2.05%) and reducing ability (35.44 ± 0.13 mM Fe2+ equivalent) at 9 mg/ml. In conclusion, the study suggests that the hydrolysate possesses the ability to manage postprandial hyperglycemia associated with diabetes mellitus via inhibition of α-amylase activity and attenuation of free radical production.

How to Cite: Babatunde, O. T., Igunnu, A., & Bewaji, C. O. (2022). In vitro α-amylase inhibitory and antioxidant properties of pancreatin-derived Polyalthia longifolia seed protein hydrolysate. Ceylon Journal of Science, 51(3), 247–257. DOI: http://doi.org/10.4038/cjs.v51i3.8032
Published on 21 Sep 2022.
Peer Reviewed

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