Research Articles
Doxorubicin-induced nephrotoxicity model in Wistar rats: Characterization of biochemical parameters, histological and immunohistochemical assessment
Authors:
Sachinthi S. Amarasiri,
University of Ruhuna, Galle, LK
About Sachinthi S.
Department of Medical Laboratory Science, Faculty of Allied Health Sciences
Anoja P. Attanayake ,
University of Ruhuna, Galle, LK
About Anoja P.
Department of Biochemistry, Faculty of Medicine
Lakmini K. B. Mudduwa,
University of Ruhuna, Galle, LK
About Lakmini K. B.
Department of Pathology, Faculty of Medicine
Kamani A. P. W. Jayatilaka
University of Ruhuna, Galle, LK
About Kamani A. P. W.
Department of Biochemistry, Faculty of Medicine
Abstract
This study characterizes the doxorubicin (DXR)-induced nephrotoxicity model in Wistar rats in terms of biochemical, histological, and immunohistochemical assessments, to provide a methodological reference model to investigate potential nephroprotective therapeutics. The experiments were carried out using four groups of healthy male Wistar rats (six rats per group) administered with a single intraperitoneal dose of normal saline (vehicle group) and DXR at 17, 20, and 23 mg/kg doses, respectively. The rats were sacrificed under anaesthesia on the seventh day after DXR administration and blood, urine, and kidney tissues were collected for investigations. The DXR dose of 17 mg/kg showed mild changes in biochemical parameters, whereas 23 mg/kg resulted in increased mortality (33%). Serum concentrations of creatinine, urea nitrogen, β2-microglobulin, urine concentrations of total protein, creatinine, and urea increased with the increased dose of DXR while serum concentrations of total protein and albumin decreased (p<0.05). Assessment of histopathology revealed the features of acute tubular injury and immunohistochemical studies revealed increased apoptosis and inflammatory changes. Based on the findings, the DXR dose of 20 mg/kg was selected as the optimal dose for the establishment of an acute nephrotoxicity model in Wistar rats to explore potential nephroprotective drug leads.
How to Cite:
Amarasiri, S. S., Attanayake, A. P., Mudduwa, L. K. B., & Jayatilaka, K. A. P. W. (2022). Doxorubicin-induced nephrotoxicity model in Wistar rats: Characterization of biochemical parameters, histological and immunohistochemical assessment. Ceylon Journal of Science, 51(4), 471–479. DOI: http://doi.org/10.4038/cjs.v51i4.8064
Published on
15 Dec 2022.
Peer Reviewed
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